Four RFLPs of the human insulin receptor gene: Pstl, Kpnl, Rsal (2 RFLPs)
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چکیده
منابع مشابه
Three RFLPs for the insulin receptor gene INSR: EcoRI, Pst I, Hind III.
SOURCE AND DESCRIPTION OF CLONE: A 973 bp cDNA insert from an adult human kidney library cloned in the EcoRI site of PBR 327 (pINSR 18.2) encoding aminoacids-26 to 295 of the insulin receptor. POLYMORPHISM EcoRI identifies a two allele polymorphism with bands at 22 fcb and 20 Kb Hind III detects two allelic fragments of 6.5 Kb and 1.4 kb Pst I reveals two allelic fragments of 2.
متن کاملTwo RFLPs at the TNP1 locus.
Source/Description: The Human Transition Protein 1 gene (TNP1) probe (1) used was a 700 bp genomic DNA sequence cloned into the Smal site of pUC8 and was supplied by W.Engel. Polymorphisms: TNP1 identifies a two allele polymorphism with TaqI: Al = 9.6 kb; A2 = 8.4 kb. Frequency: Analysis of 20 unrelated individuals yielded frequencies: Al = 0.23; A2 = 0.77. Rsal in a screen of 10-20 unrelated i...
متن کاملTwo RFLPs in human inter-alpha-trypsin inhibitor heavy chain gene ITIH2 on chromosome 10.
SOURCE AND DESCRIPTION OF CLONE : The 0.8 kb Eco RI / Bam HI fragment of lambda HuHITI-9 (1) used as a probe codes for human heavy chain H2 of the inter-alpha-trypsin inhibitor (2). POLYMORPHISMS : Kpn 1 (GGTAC/C) identifies one invariant band at 8.5 kb and a diaJlelic polymorphism with DNA fragments at 26.0 kb or 20.0 kb. Msp I (C/CGG) identifies three invariant bands at 2.35 kb, 2.1 kb and 1....
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Sixty-nine unrelated Chinese DMD patients were studied with a series of genomic and cDNA probes. Analysis of 13 polymorphic sites showed that pERT87-1, 87-8, 87-15, and XJ probes gave favourable allele frequencies in the Chinese population, and nearly 90% of the DMD families in this study were informative for prenatal diagnosis and carrier detection using these four polymorphic markers. Nine ou...
متن کاملPhylogenetic relationships in the genus Oryza based on mitochondrial RFLPs.
Restriction fragment length polymorphism (RFLP) of mitochondrial DNA in the genus Oryza was surveyed using 20 accessions including 11 species and a single endonuclease, EcoRI. RFLPs were visualized by Southern hybridization with eight rice mitochondrial DNA probes labeled non-radioactively with digoxigenin-dUTP. A total of 66 bands were obtained from all of the accessions. The total number of f...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.2.820